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Methods Experimental animals Three strains of male rats were included in this study: Sprague-Dawley (SD) rats, Wistar Kyoto (WKY) rats, and Spontaneously Hypertensive (SHR) rats

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1 Validated Salt Stoichiometry : Many grey-market vendors confuse the Cation MW (159.21) with the Salt MW (286.11), leading to massive titration variance (calculation errors) in stock solutions

Compared with gut microbiota, gastric microbiota is characterized by low density, poor specificity, and large fluctuations