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Li J, Zhao S, Zhou X, Zhang T, Zhao L, Miao P et al

9M, incubation with CM from the vector adipocytes enhanced the phosphorylation of IKK/, IB, and p65, suggesting activation of NF-B signaling pathway, which was partially reversed by CM from the SIRT3OE-CPT2KD adipocytes

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This aligns with observations from comparative extraction studies showing that partial ethanol extraction can cause protein loss, whereas an optimized method such as the supercritical carbon dioxide-ethanol produced higher protein recovery (Senarathna & Malalgoda, 2024)