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The day before the assay, frozen aliquots of the above-mentioned BHK cell lines were thawed, washed once in PBS (Gibco, #14190-094) and seeded in 40 L medium in a white 384-well culture plate (PerkinElmer, #6007688) at a cell density of 4000 cells/well and incubated in plastic bags over night at 37 C and 5% CO 2

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ARA-290 was well-tolerated in clinical trials with no serious adverse events

Johansson et al

Improved Skin Density and Firmness: Clinical trials demonstrate enhanced skin laxity, clarity, and firmness, particularly in photoaged skin