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N-acetyl-L-cysteine plays a crucial role in the synthesis of glutathione, a powerful endogenous antioxidant that helps neutralize free radicals

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The Preiss-Handler pathway synthesizes NAD + from nicotinic acid (Niacin) The salvage pathway recycles nicotinamide/niacinamide to nicotinamide mononucleotide (NMN) by NAMPT and NMN is converted into NAD+, and in this pathway nicotinamide riboside (NR) converts into NMN

Subsequently, Image J software was employed for quantitative analysis of the fluorescence signals to evaluate and compare the differences in the distribution of Poly-A + RNA between the nucleus and cytoplasm under the two treatment conditions 59

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