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Glutathione is a small compound made inside the body that helps protect cells from oxidative wear

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To fully phosphorylate the C-terminal extension of UBE2H for ubiquitylation assays (which boosts its CTLH E3-dependent activity), it was coexpressed with the catalytic subunit of the kinase CK2 (CK26His) 39 and subjected to anion-exchange chromatography with the HiTrap Q HP column (Cytiva) before final SEC in buffer A