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Pellets were thawed and resuspended in purification buffer (150 mM NaCl, 2 mM MgCl 2 , 50 mM HEPES-KOH pH 7.5, 5 mM -mercaptoethanol, 10% (v/v) glycerol) supplemented with protease inhibitors and DNase I before lysis by sonication

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Magnesium: Magnesium participates in more than 300 reactions in the body and is essential for nerve, muscle and heart function

However, discontinuation or interruption is clearly indicated for certain serious syndromes in product labelling (for example, suspected pancreatitis