Most peptides degrade in the digestive system, making injectable administration standard
The GST construct (Amersham Biosciences) in pGEX-4T-3 vector was purified by manual glutathione-affinity chromatography using Glutathione Sepharose 4 fast flow beads (#17-5132-01, GE Healthcare) and eluted with 10 mM reduced glutathione (#11514, SRL), 150 mM NaCl and 2 mM DTT
Morinaga Low Fat High Protein Skim Milk Collagen Powder Morinaga Collagen Skim Milk Powder is a milk powder fortified with extra protein, 1000 mg of collagen, 10 billion lactic acid bacteria, iron, and vitamin C, all in one 20g serving
The muscle of the upper arm (or the thigh or glute, alternatively) is injected with ~1mL (less than a full syringe) of B12
Im especially grateful for the chance to deepen my understanding of structural inequities in health, and to contribute to work that uplifts marginalized communities through rigorous, justice-centered public health research