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MRS data were collected using a 1 H-MRS single voxel short TE STEAM (STimulated Echo Acquisition Mode) sequence (TE/TM/TR = 17/17/2000 ms) with eight phase cycle steps, 4096 samples, and a 4 kHz bandwidth

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Immunoprecipitation To enrich PDHc-E2 or OGDHc-E2 for proteomics analysis, 5 10 6 cells were lysed in 1.5 ml IP buffer (50 mM HEPES (pH 7.0), 150 mM NaCl, 1% (vol/vol) IGEPAL CA-630, 1 mM phenylmethylsulfonyl fluoride, 10 mM Tris(2-carboxyethyl)phosphine hydrochloride, 100 mM nicotinamide, 1 Roche Complete Protease Inhibitor Cocktail) and rotated at 4 C for 30 min

In conclusion, exhaustive quality control testing of the final product is indispensable to ensure it is free of hazardous contaminants, including pathogenic microbes, mycotoxins, and heavy metals (544)