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Proteins were next transferred to polyvinylidene difluoride (PVDF) membranes and then blocked in milk blocking buffer (5% non-fat dry milk in 13.7 mM NaCl, 0.27 mM KCl, 0.2 mM KH 2 PO 4 , 1 mM Na2HPO 4 , 0.1% Tween 20) at room temperature for 1 h

The promising results of this technique will be presented