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Mobile phases were A: MilliQ water (0.1% formic acid) and B: 100% methanol

Fish Physiol Biochem

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The constant levels of marker proteins such as GAPDH, combined with observations indicating that knockdown of the UPR element, BiP/Grp78 , revealed no detectable effects on Nrf2, GCLC, or glutathione, further support the conclusion that NFT-mediated stabilization of Nrf2 is a major contributor to its accumulation and the subsequent stimulation of GCL and glutathione synthesis

It's present throughout your dog's body