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Neuropathological studies have shown that CAA-associated microinfarcts colocalize with phosphorylated tau in the neocortex, accelerating Tau tangle formation through activation of glycogen synthase kinase-3 (GSK-3) 58

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Western blot analysis To quantify the protein samples, tissue homogenates and cells were homogenized and quantified using a BCA kit (Beyotime, Shanghai, China)