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Structural Biophysical Analysis: Identification of key electrostatic interactions (salt bridges and hydrogen bonds) at the binding interface using distance cutoffs of 4.0 for salt bridges, and a cutoff value of the angle formed by acceptor (A), donor (D) and hydrogen (H) (ADH) of 30 and donor-acceptor (D-A) distance at 3.0 for hydrogen bonds

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[9] [10] Classification [edit] Protein sequence and structure are important additional classification criteria for the three superfamilies (cytosolic, mitochondrial, and MAPEG) of GSTs: while classes from the cytosolic superfamily of GSTs possess more than 40% sequence homology, those from other classes may have less than 25%

However, smoking specifically increases the risk of breast cancer by upregulating the CYP1B1 and CYP1A1 enzymes