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Obesity is a leading risk for higher medical costs, disability claims, absenteeism, and productivity loss

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Furthermore, some non-classical MQC pathways can be regulated via direct mitochondria-lysosome contacts

These off-target interactions can influence distribution, metabolism, and toxicity

When the TSA method was used, the sections were treated with biotinylated goat anti-mouse/rabbit IgG (1:1000 dilution, Vector Laboratories, Burlingame, CA) for 1 h, and then incubated with HRP-conjugated-avidin for 30 min (1:100 dilution in TN buffer, TSA System) at room temperature