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Lipid peroxidation through accumulation of malondialdehyde (MDA) was determined by homogenizing the tissue in ice-cold mixture of 20% (v/v) TCA containing 0.5% thio barbituric acid (TBA) (1:1)
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First, the tryptophan residue at position 1 and the peptide bonds throughout the chain are substrates for plasma peptidases, which accounts for its short half-life
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