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doi:10.1159/000109459

DP cells will slowly overflow in DMEM/F-12 (Dulbeccos Modified Eagle Medium/Nutrient Mixture F-12), 20% (v/v) fetal bovine serum (FBS) supplemented with 100 units/ml penicillin and 0.1 mg/ml streptomycin (Invitrogen, Carlsbad, CA, and Sigma, St